complementary dna libraries (10X Genomics)

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10X Genomics complementary dna libraries
Complementary Dna Libraries, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/complementary-dna+libraries/complementary+dna+libraries/pm42156893-258-7-15
Average 86 stars, based on 1 article reviews

complementary dna libraries - by Bioz Stars, 2026-11

86/100 stars

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cDNA Library Assay:

Article Title: Identification of glutamine as a potential therapeutic target in dry eye disease
Article Snippet: .. The complementary deoxyribonucleic acid (cDNA) library was generated using a commercial 10x Genomics platform (10x Genomics, Pleasanton, CA, USA). .. Single-cell transcriptome amplification and library preparation were performed using the Single-Cell 3’ Library Kit v3 (10x Genomics) by Capitalbio Technology Corporation according to manufacturer’s instructions.

Article Title: Patient-Derived Organoids from Multiple Sites of a Single Tumor Recapitulates Intratumoral Heterogeneity in Patients with Gastric Cancer.
Article Snippet: .. We utilized the 10X Genomics Chromium Instrument and cDNA synthesis kit (10x Genomics: Chromium Next GEM Single Cell 3 ́ Library and Gel Bead Kit v3.1; Pleasanton, CA, USA) to generate a barcoded cDNA library for single-nucleus RNA sequencing from https://doi.org/10.5009/gnl250108 3 sorted nuclei. cDNA library quality was determined using an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Using this library, we ran two paired-end 200bp flow cells on an Illumina NovaSeq 5000/6000 S1 Reagent Kit v1.5 (200 cycles) (read lengths: 28 bp Read1, 10 bp I7 Index, 10 bp I5 Index, and 90 bp Read2; Illumina, San Diego, CA, USA).

Generated:

Article Title: Early sex-related transcriptional differences in CD8 + T cells responding to chronic viral infection reveal a sex bias in exhaustion
Article Snippet: .. Illumina Single Cell 3’ complementary DNA (cDNA) libraries were generated of all groups were generated in parallel according to the manufacturer’s instructions (10x Genomics). .. Quality control measures of the single-cell cDNA libraries were performed on the 2100 Bioanalyzer (Agilent Technologies) and Qubit 4.0 Fluorometer (Thermo Fisher Scientific).

Extraction:

Article Title: Adenophages are an atypical macrophage population in exocrine glands sustained by ILC2-derived GM-CSF.
Article Snippet: Frederike Westermann, Selma Tuzlak, Victor Kreiner , Aline Ignacio , David Bejarano , Mitchell Bijnen , Virginia Cecconi, Hannah van Hove , Haiting Wang, Myrto Andreadou, Gioana Litscher, Colin Sparano , Ricardo Fróis-Martins , Alexandre Gallerand , Elsa Roussel, Laura Oberbichler , Rachel Lindemann , Donatella DeFeo , Zhaoyuan Liu, Anja Kipar , Salomé LeibundGut-Landmann , Kathy McCoy , Iain Nixon, Calum C. Bain , Christoph Schneider , Stoyan Ivanov , Sonia Tugues , Melanie Greter , Florent Ginhoux , Andreas Schlitzer , Elaine Emmerson & Burkhard Becher

Single Cell:

Article Title: RANBP1 promotes oral cancer progression through activating YAP1 and NDUFB3 derived from the database of single-cell sequencing.
Article Snippet: .. Subsequent steps comprising reverse 460 transcription and cDNA amplification were conducted to generate 3’-single-cell cDNA 461 libraries (ChromiumTM single cell 3’ v3.1 reagent kit, 10x Genomics). .. The libraries 462 generated were sequenced using NovaSeq 6000 Sequencing System (Illumina).

DNA Library Preparation:

Amplification:

cDNA Synthesis:

RNA Sequencing:

other:

Article Title: Single cell transcriptomics in blood of patients with chronic obstructive pulmonary disease
Article Snippet: We utilized the 10X Genomics Chromium Instrument and copy DNA (cDNA) synthesis kit (10x Genomics: Chromium Next GEM Automated Single Cell 5′ Library, Gel Bead Kit v1.1 and Human TCR/BCR amplification kit) to generate a barcoded cDNA library for scRNA-seq from approximately 3,000 cells.

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